Crystallization and preliminary X-ray studies of human recombinant interleukin-2.

نویسندگان

  • C Sano
  • K Ishikawa
  • N Nagashima
  • T Tsuji
  • T Kawakita
  • K Fukuhara
  • Y Mitsui
  • Y Iitaka
چکیده

Two different forms of crystals (potentially) suitable for x-ray structure analysis were obtained for recombinant human interleukin-2 (IL-2) using ammonium sulfate as a precipitant in the pH range of 6.3-7.3 (in the case of hexagonal bipyramidal crystals) and 4.5-5.5 (in the case of plate crystals). The hexagonal bipyramidal crystal belongs to a hexagonal space group P6(2)22 or P6(4)22 with a = b = 105.8 A and c = 122.2 A. The crystal diffracts up to 3.4 A resolution and contains 2 or 3 IL-2 molecules in an asymmetric unit. The plate crystal belongs to an orthorhombic space group P2(1)2(1)2 with a = 47.9 A, b = 79.6 A, and c = 31.9 A. The crystal diffracts up to 2.5 A resolution and contains only 1 IL-2 molecule in an asymmetric unit. These facts reconfirmed crystallographically the high homogeneity of the present preparation of human recombinant IL-2.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 262 10  شماره 

صفحات  -

تاریخ انتشار 1987